产品货号：
GS4743
中文名称：
重组人MIF(带His标签)
英文名称：
Recombinant Human Migration Inhibitor Factor, His
产品规格：
10μg
发货周期：
1～3天
产品价格：
询价
分子量：Approximately 13.5kDa,a single non-glycosylated polypeptide chain containing 117 amino acids,with 6×His at the C-terminus.
储存条件：冷冻（-20℃）
概述
Migration Inhibitory Factor(MIF)is a secreted protein without a cleavable signal sequence and is secreted via a specialized,non-classical pathway.It is secreted by macrophages upon stimulation by bacterial lipopolysaccharide(LPS),or by M.tuberculosis antigens.MIF consists of two α-helices and six β-strands,four of which form a β-sheet.The two remaining β-strands interact with other MIF molecules,creating a trimer.Structure-function studies suggest MIF is bifunctional with segregated topology.The N- and C-termini mediate enzyme activity(in theory).Phenylpyruvate tautomerase activity(enol-to-keto)has been demonstrated and is dependent upon Pro at position 1.Amino acids 50-65(a.a.)have also been suggested to contain thiol-protein oxidoreductase activity.MIF has proinflammatory cytokine activity centered around 49 - 65(a.a.).On fibroblasts,MIF induces,IL-1,IL-8 and MMP expression;on macrophages,MIF stimulates NO production and TNF-α release folllowing IFN-γ activation.MIF apparently acts through CD74 and CD44,likely in some form of trimeric interaction.Human MIF is active on mouse cells.Human MIF is 90%,94%,95%,and 90%a.a.identical to mouse,bovine,porcine and rat MIF,respectively.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
相关搜索：重组人MIF(带His标签)，MIF, His-tagged, Human，Recombinant Human Migration Inhibitor Factor, His
储存条件：冷冻（-20℃）
概述
Migration Inhibitory Factor(MIF)is a secreted protein without a cleavable signal sequence and is secreted via a specialized,non-classical pathway.It is secreted by macrophages upon stimulation by bacterial lipopolysaccharide(LPS),or by M.tuberculosis antigens.MIF consists of two α-helices and six β-strands,four of which form a β-sheet.The two remaining β-strands interact with other MIF molecules,creating a trimer.Structure-function studies suggest MIF is bifunctional with segregated topology.The N- and C-termini mediate enzyme activity(in theory).Phenylpyruvate tautomerase activity(enol-to-keto)has been demonstrated and is dependent upon Pro at position 1.Amino acids 50-65(a.a.)have also been suggested to contain thiol-protein oxidoreductase activity.MIF has proinflammatory cytokine activity centered around 49 - 65(a.a.).On fibroblasts,MIF induces,IL-1,IL-8 and MMP expression;on macrophages,MIF stimulates NO production and TNF-α release folllowing IFN-γ activation.MIF apparently acts through CD74 and CD44,likely in some form of trimeric interaction.Human MIF is active on mouse cells.Human MIF is 90%,94%,95%,and 90%a.a.identical to mouse,bovine,porcine and rat MIF,respectively.
使用说明
This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
技术规格
| 外观 | Sterile Filtered White lyophilized(freeze-dried)powder. |
| 纯度 | ＞95%by SDS-PAGE and HPLC analyses. |
| 来源 | Escherichia coli. |
| 生物活性 | Fully biologically active when compared to standard.The specific activity is determined by binding rhCD74 in a functional ELISA. |
| 成分 | Lyophilized from a 0.2μmfiltered concentrated solution in PBS,pH 7.4. |
| 内毒素 | Less than 1 EU/μg of rHuMIF,His as determined by LAL method. |
| 复溶 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃.Further dilutions should be made in appropriate buffered solutions. |
| 氨基酸序列 | MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY DMNAANVGWN NSTFALEHHH HHH |
相关搜索：重组人MIF(带His标签)，MIF, His-tagged, Human，Recombinant Human Migration Inhibitor Factor, His